Consequence of Absence of Nitrate Reductase Activity on Photosynthesis in Nicotiana plumbaginifolia Plants
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چکیده
منابع مشابه
Consequence of Absence of Nitrate Reductase Activity on Photosynthesis in Nicotiana plumbaginifolia Plants.
Chlorate-resistant Nicotiana plumbaginifolia (cv Viviani) mutants were found to be deficient in the nitrate reductase apoprotein (NR(-)nia). Because they could not grow with nitrate as sole nitrogen source, they were cultivated as graftings on wild-type Nicotiana tabacum plants. The grafts of mutant plants were chlorotic compared to the grafts of wild type. Mutant leaves did not accumulate nitr...
متن کاملMutational and structural analysis of the nitrate reductase heme domain of Nicotiana plumbaginifolia.
We have analyzed four Nicotiana plumbaginifolia null mutants presumably affected in the heme domain of nitrate reductase. The DNA sequence of this domain has been determined for each mutant and for the wild type. Two mutations were identified as single base changes leading to, respectively, the substitution of a histidine residue by an asparagine (mutant E56) and to the appearance of an ochre s...
متن کاملAnalysis of nitrate reductase mRNA expression and nitrate reductase activity in response to nitrogen supply
Nitrate is one of the major sources of nitrogen for the growth of plants. It is taken up by plant roots and transported to the leaves where it is reduced to nitrite in the. The main objective of this research was to investigate stimulatory effects of sodium nitrate, potassium nitrate, ammonia and urea on the production/generation of the nitrate reductase mRNA in Triticum aestivum plants. The pl...
متن کاملEffect of AtNRT2.1 transgene on HATS nitrate uptake in transgenic Nicotiana plumbaginifolia
To investigate the impact of overexpression of AtNRT2.1 transgene from Arabidopsis on nitrate uptake rate and to understand the regulation of endogenous HATS by nitrate and glutamine amino acid (Gln) in tobacco plants, wild-type and transgenic (F line) plants grown on soil for 4 weeks were transferred to hydroponic culture in a controlled-environment with a 16/8h L:D photoperiod at 24? C/20...
متن کاملGenetic and biochemical analysis of intragenic complementation events among nitrate reductase apoenzyme-deficient mutants of Nicotiana plumbaginifolia.
Intragenic complementation has been observed between apoenzyme nitrate reductase-deficient mutants (nia) of Nicotiana plumbaginifolia. In vivo as in vitro, the NADH-nitrate reductase (NR) activity in plants heterozygous for two different nia alleles was lower than in the wild type plant, but the plants were able to grow on nitrate as a sole nitrogen source. NR activity, absent in extracts of ho...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1987
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.84.1.67